Review



caspase‐3,7 substrate (ac‐devd‐afc  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Millipore caspase‐3,7 substrate (ac‐devd‐afc
    Caspase‐3,7 Substrate (Ac‐Devd‐Afc, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/caspase+3+7+substrate++ac+devd+afc/pm37797174-439-39-50
    Average 90 stars, based on 1 article reviews
    caspase‐3,7 substrate (ac‐devd‐afc - by Bioz Stars, 2026-11
    90/100 stars

    Images

    Related Articles

    Activity Assay:

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: p53 directs focused genomic responses in Drosophila.
    Article Snippet: Five micrograms of a protein extract was incubated with 10 mM Ac-DEVD-AFC (Calbiochem, La Jolla, CA, USA) substrate in a final volume of 100 ml in a 96-well microtiter plate.

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: Caspase 3 Activity Assay MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase Activity Assay Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Nanoparticles carrying nucleic acid cassettes for expressing RNA
    Article Snippet: Caspase 3 Activity Assay:MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, Calif.).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Generated:

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: p53 directs focused genomic responses in Drosophila.
    Article Snippet: Five micrograms of a protein extract was incubated with 10 mM Ac-DEVD-AFC (Calbiochem, La Jolla, CA, USA) substrate in a final volume of 100 ml in a 96-well microtiter plate.

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: Caspase 3 Activity Assay MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase Activity Assay Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Nanoparticles carrying nucleic acid cassettes for expressing RNA
    Article Snippet: Caspase 3 Activity Assay:MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, Calif.).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Transfection:

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: p53 directs focused genomic responses in Drosophila.
    Article Snippet: Five micrograms of a protein extract was incubated with 10 mM Ac-DEVD-AFC (Calbiochem, La Jolla, CA, USA) substrate in a final volume of 100 ml in a 96-well microtiter plate.

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: Caspase 3 Activity Assay MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase Activity Assay Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Nanoparticles carrying nucleic acid cassettes for expressing RNA
    Article Snippet: Caspase 3 Activity Assay:MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, Calif.).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Irradiation:

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: p53 directs focused genomic responses in Drosophila.
    Article Snippet: Five micrograms of a protein extract was incubated with 10 mM Ac-DEVD-AFC (Calbiochem, La Jolla, CA, USA) substrate in a final volume of 100 ml in a 96-well microtiter plate.

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: Caspase 3 Activity Assay MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase Activity Assay Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Nanoparticles carrying nucleic acid cassettes for expressing RNA
    Article Snippet: Caspase 3 Activity Assay:MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, Calif.).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Caspase-3 Activity Assay:

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: p53 directs focused genomic responses in Drosophila.
    Article Snippet: Five micrograms of a protein extract was incubated with 10 mM Ac-DEVD-AFC (Calbiochem, La Jolla, CA, USA) substrate in a final volume of 100 ml in a 96-well microtiter plate.

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: Caspase 3 Activity Assay MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase Activity Assay Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Nanoparticles carrying nucleic acid cassettes for expressing RNA
    Article Snippet: Caspase 3 Activity Assay:MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, Calif.).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Caspase Activity Assay:

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: p53 directs focused genomic responses in Drosophila.
    Article Snippet: Five micrograms of a protein extract was incubated with 10 mM Ac-DEVD-AFC (Calbiochem, La Jolla, CA, USA) substrate in a final volume of 100 ml in a 96-well microtiter plate.

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Site-Specific Promoter Caging Enables Optochemical Gene Activation in Cells and Animals
    Article Snippet: HeLa cells were transfected with the noncaged or caged EGFP-Plk3 plasmids and either kept in the dark or irradiated (365 nm, 5 min) and caspase-3 activity was measured after 48 h (Ac-DEVD-AFC substrate, Calbiochem).

    Article Title: Targeted Delivery of siRNA-Generating DNA Nanocassettes Using Multifunctional Nanoparticles
    Article Snippet: Caspase 3 Activity Assay MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase Activity Assay Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).

    Article Title: Nanoparticles carrying nucleic acid cassettes for expressing RNA
    Article Snippet: Caspase 3 Activity Assay:MIA PaCa-2 cells were treated with various nanoparticles in the presence or absence of gemcitabine for 48 h. Cell lysates were analyzed for their levels of caspase 3-like activity, which is generated by caspases 3, 7, and 10, using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, Calif.).

    Article Title: Adiponectin Modulates C-Jun N-Terminal Kinase and Mammalian Target of Rapamycin and inhibits hepatocellular carcinoma
    Article Snippet: Caspase-3 like activity was detected in cell lysates after various treatments using an Ac-DEVD-AFC substrate (Calbiochem, San Diego, CA).



    Similar Products

    94
    MedChemExpress casp substrates ac devd afc
    Casp Substrates Ac Devd Afc, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/Ac-DEVD-AFC/10__1016_slash_j__aquaculture__2025__742828-123-1-10
    Average 94 stars, based on 1 article reviews
    casp substrates ac devd afc - by Bioz Stars, 2026-11
    94/100 stars
      Buy from Supplier

    90
    Bachem substrate ac-devd-afc #4027914
    Substrate Ac Devd Afc #4027914, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/substrate+ac+devd+afc++4027914/pm40331965-295-1-6
    Average 90 stars, based on 1 article reviews
    substrate ac-devd-afc #4027914 - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Biomol GmbH chromogenic peptide substrate cleavage ac-devd-afc
    Chromogenic Peptide Substrate Cleavage Ac Devd Afc, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/fluorogenic+substrate+ac+devd+amc/pmc11608749-69-6-11
    Average 90 stars, based on 1 article reviews
    chromogenic peptide substrate cleavage ac-devd-afc - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Cayman Chemical ac-devd-afc substrate
    Ac Devd Afc Substrate, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+afc+substrate/pm38716700-304-30-31
    Average 90 stars, based on 1 article reviews
    ac-devd-afc substrate - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    AAT Bioquest ac-devd-afc substrate
    Ac Devd Afc Substrate, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+afc+substrate/pm38688061-82-20-24
    Average 90 stars, based on 1 article reviews
    ac-devd-afc substrate - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Enzo Biochem ac-devd-afc substrate
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Ac Devd Afc Substrate, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+afc+substrate/bio_rxiv__2023__12__12__571243-132-14-16
    Average 90 stars, based on 1 article reviews
    ac-devd-afc substrate - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Millipore caspase‐3,7 substrate (ac‐devd‐afc
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Caspase‐3,7 Substrate (Ac‐Devd‐Afc, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/caspase+3+7+substrate++ac+devd+afc/pm37797174-439-39-50
    Average 90 stars, based on 1 article reviews
    caspase‐3,7 substrate (ac‐devd‐afc - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Cayman Chemical ac-devd-afc caspase-3 substrate
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Ac Devd Afc Caspase 3 Substrate, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/ac+devd+amc+fluorogenic+substrate/pmc10294886-46-29-30
    Average 90 stars, based on 1 article reviews
    ac-devd-afc caspase-3 substrate - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Tocris caspase substrates ac-devd-afc
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Caspase Substrates Ac Devd Afc, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/caspase+substrates+ac+devd+afc/pm36982628-415-7-15
    Average 90 stars, based on 1 article reviews
    caspase substrates ac-devd-afc - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    90
    Biomol GmbH ac-devd-afc fluorogenic substrate
    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, <t>H)</t> <t>Caspase-3</t> activity determined using <t>Ac-DEVD-AFC</t> as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.
    Ac Devd Afc Fluorogenic Substrate, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ac-devd-afc+substrate/fluorogenic+substrate+ac+devd+amc/pmc03546013-207-7-10
    Average 90 stars, based on 1 article reviews
    ac-devd-afc fluorogenic substrate - by Bioz Stars, 2026-11
    90/100 stars
      Buy from Supplier

    Image Search Results


    Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Journal: bioRxiv

    Article Title: Blocking Orai1 constitutive activity inhibits B-cell cancer migration and synergistically acts with drugs to reduce B-CLL cell survival

    doi: 10.1101/2023.12.12.571243

    Figure Lengend Snippet: Primary B-CLL cells (A, B) and B-cell cancer lines (C, D) treated with GSK-7975A (GSK, 10, 20, 50 μM) for 48 hours. Alive and dead cells were determined using LIVE/DEAD® staining Kits and analyzed by flow cytometry. (A, C) Representative flow cytometry plots. (B, D) Quantification of the percentage of dead cells in (A, C). Cell mortality was also determined by flow cytometry after annexin V/PI staining (E, F). The percentage of total apoptotic cells was calculated as the percentage of annexin V-FITC positive plus annexin V-FITC/PI-positive population. (G, H) Caspase-3 activity determined using Ac-DEVD-AFC as substrate after 24 hours with GSK-7975A (GSK, 0-50 μM) or Synta66 (S66, 0-50 μM) in primary B-CLL cells and B-cell cancer lines. Data from B-cell cancer lines are representative of at least 3 independent experiments. Statistical significance was analyzed by two-tailed, unpaired Student’s t test.

    Article Snippet: As reported and described previously [ ], activity of caspase-3 was performed by using Ac-DEVD-AFC substrate (Enzo Life Sciences, France).

    Techniques: Staining, Flow Cytometry, Activity Assay, Two Tailed Test